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michael davidson  (Addgene inc)


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    Addgene inc michael davidson
    Michael Davidson, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/michael davidson/product/Addgene inc
    Average 92 stars, based on 5 article reviews
    michael davidson - by Bioz Stars, 2026-06
    92/100 stars

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    Microtubules are nucleated at specific sites within the Golgi ribbon. (a) An RPE1 cell <t>expressing</t> <t>Emerald-EB3</t> (green, MT <t>+TIP</t> marker) and TGN-RFP (magenta, Golgi marker). A maximum-intensity projection of a confocal spinning disk microscopy sequence over a 3-min period and Z thickness 3 μm is shown (a, a′). Inset in a is enlarged in a′, showing newly formed GDMTs formed at the same site (arrows); asterisk indicates centrosome position. (b) Quantification of nearest-neighbor distance between GDMT nucleation sites, based on 3D live-time imaging (Reproduced from [9] with permission from [MBoC])
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    Microtubules are nucleated at specific sites within the Golgi ribbon. (a) An RPE1 cell expressing Emerald-EB3 (green, MT +TIP marker) and TGN-RFP (magenta, Golgi marker). A maximum-intensity projection of a confocal spinning disk microscopy sequence over a 3-min period and Z thickness 3 μm is shown (a, a′). Inset in a is enlarged in a′, showing newly formed GDMTs formed at the same site (arrows); asterisk indicates centrosome position. (b) Quantification of nearest-neighbor distance between GDMT nucleation sites, based on 3D live-time imaging (Reproduced from [9] with permission from [MBoC])

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Detection of Microtubule Nucleation Hotspots at the Golgi

    doi: 10.1007/978-1-0716-0219-5_12

    Figure Lengend Snippet: Microtubules are nucleated at specific sites within the Golgi ribbon. (a) An RPE1 cell expressing Emerald-EB3 (green, MT +TIP marker) and TGN-RFP (magenta, Golgi marker). A maximum-intensity projection of a confocal spinning disk microscopy sequence over a 3-min period and Z thickness 3 μm is shown (a, a′). Inset in a is enlarged in a′, showing newly formed GDMTs formed at the same site (arrows); asterisk indicates centrosome position. (b) Quantification of nearest-neighbor distance between GDMT nucleation sites, based on 3D live-time imaging (Reproduced from [9] with permission from [MBoC])

    Article Snippet: Plasmids: As MT +TIP markers, Emerald-EB3 (kind gift from M. Davidson; Addgene plasmid # 54076) or another fluorescently tagged +TIP marker can be used.

    Techniques: Expressing, Marker, Microscopy, Sequencing, Imaging